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hcc 70 crl 2315 human breast cancer cell lines  (ATCC)


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    ATCC hcc 70 crl 2315 human breast cancer cell lines
    Hcc 70 Crl 2315 Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcc 70 crl 2315 human breast cancer cell lines/product/ATCC
    Average 96 stars, based on 493 article reviews
    hcc 70 crl 2315 human breast cancer cell lines - by Bioz Stars, 2026-05
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    ABHD12 is highly expressed in breast cancer tissues and promotes the proliferative capacity of breast cancer cells. (A and B) WB experiments confirm the expression of ABHD12 protein in breast cancer tissues and adjacent normal breast tissues. (C and D) IHC experiments verify the expression and localization of ABHD12 in breast cancer tissues (cytoplasm). ( E ) RT-qPCR experiments confirm the expression of ABHD12 mRNA in normal breast cells and breast cancer cells. Verification of ABHD12 knockdown and overexpression efficiency at the mRNA level in (F) MDA-MB-231 cell line and ( G <t>)</t> <t>HCC-1937</t> cell line. ( H ) Verification of ABHD12 knockdown and overexpression protein efficiency at protein level in MDA-MB-231 and HCC-1937 cell line. ( I ) Colony formation assays and their quantitative analysis in ( J ) MDA-MB-231 and ( K ) HCC-1937 cell lines further validate the impact of ABHD12 on cell proliferation. CCK-8 experiments demonstrate the effects of different ABHD12 expression levels on the proliferative capacity of ( L ) MDA-MB-231 cell line and ( M ) HCC-1937 cell line. * p <0.05, ** p <0.01, *** p <0.001; ns indicates no statistical significance.
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    ABHD12 is highly expressed in breast cancer tissues and promotes the proliferative capacity of breast cancer cells. (A and B) WB experiments confirm the expression of ABHD12 protein in breast cancer tissues and adjacent normal breast tissues. (C and D) IHC experiments verify the expression and localization of ABHD12 in breast cancer tissues (cytoplasm). ( E ) RT-qPCR experiments confirm the expression of ABHD12 mRNA in normal breast cells and breast cancer cells. Verification of ABHD12 knockdown and overexpression efficiency at the mRNA level in (F) MDA-MB-231 cell line and ( G ) HCC-1937 cell line. ( H ) Verification of ABHD12 knockdown and overexpression protein efficiency at protein level in MDA-MB-231 and HCC-1937 cell line. ( I ) Colony formation assays and their quantitative analysis in ( J ) MDA-MB-231 and ( K ) HCC-1937 cell lines further validate the impact of ABHD12 on cell proliferation. CCK-8 experiments demonstrate the effects of different ABHD12 expression levels on the proliferative capacity of ( L ) MDA-MB-231 cell line and ( M ) HCC-1937 cell line. * p <0.05, ** p <0.01, *** p <0.001; ns indicates no statistical significance.

    Journal: Breast Cancer : Targets and Therapy

    Article Title: Multi-Omics Characterization of ABHD12 Across Pan-Cancer and Validation of Its Role in Promoting Proliferation and Metastasis in Breast Cancer

    doi: 10.2147/BCTT.S554836

    Figure Lengend Snippet: ABHD12 is highly expressed in breast cancer tissues and promotes the proliferative capacity of breast cancer cells. (A and B) WB experiments confirm the expression of ABHD12 protein in breast cancer tissues and adjacent normal breast tissues. (C and D) IHC experiments verify the expression and localization of ABHD12 in breast cancer tissues (cytoplasm). ( E ) RT-qPCR experiments confirm the expression of ABHD12 mRNA in normal breast cells and breast cancer cells. Verification of ABHD12 knockdown and overexpression efficiency at the mRNA level in (F) MDA-MB-231 cell line and ( G ) HCC-1937 cell line. ( H ) Verification of ABHD12 knockdown and overexpression protein efficiency at protein level in MDA-MB-231 and HCC-1937 cell line. ( I ) Colony formation assays and their quantitative analysis in ( J ) MDA-MB-231 and ( K ) HCC-1937 cell lines further validate the impact of ABHD12 on cell proliferation. CCK-8 experiments demonstrate the effects of different ABHD12 expression levels on the proliferative capacity of ( L ) MDA-MB-231 cell line and ( M ) HCC-1937 cell line. * p <0.05, ** p <0.01, *** p <0.001; ns indicates no statistical significance.

    Article Snippet: BRCA cell lines HCC-1937, MCF-10A, MCF-7, BT474, SK-BR-3, MDA-MB-468, BT-549, and MDA-MB-231 were purchased from Procell Life Science & Technology Co., Ltd., Wuhan, China.

    Techniques: Expressing, Quantitative RT-PCR, Knockdown, Over Expression, CCK-8 Assay

    Overexpression of ABHD12 in breast cancer cell lines enhances the invasive and migratory capabilities of breast cancer cells and promotes tumor formation in mice. Scratch assays demonstrate the differential effects of varying ABHD12 expression levels on the migratory capacity of (A and B) MDA-MB-231 cell line and (C and D) HCC-1937 cell line. Transwell invasion and migration assays validate the impact of ABHD12 knockdown and overexpression on the invasive and migratory properties of ( E–G ) MDA-MB-231 cell line and ( H–J ) HCC-1937 cell line. ( K–N ) Animal experiments assess the effects of ABHD12 knockdown and overexpression on body weight and tumor mass/volume in NCG mice. * p <0.05, ** p <0.01, *** p <0.001.

    Journal: Breast Cancer : Targets and Therapy

    Article Title: Multi-Omics Characterization of ABHD12 Across Pan-Cancer and Validation of Its Role in Promoting Proliferation and Metastasis in Breast Cancer

    doi: 10.2147/BCTT.S554836

    Figure Lengend Snippet: Overexpression of ABHD12 in breast cancer cell lines enhances the invasive and migratory capabilities of breast cancer cells and promotes tumor formation in mice. Scratch assays demonstrate the differential effects of varying ABHD12 expression levels on the migratory capacity of (A and B) MDA-MB-231 cell line and (C and D) HCC-1937 cell line. Transwell invasion and migration assays validate the impact of ABHD12 knockdown and overexpression on the invasive and migratory properties of ( E–G ) MDA-MB-231 cell line and ( H–J ) HCC-1937 cell line. ( K–N ) Animal experiments assess the effects of ABHD12 knockdown and overexpression on body weight and tumor mass/volume in NCG mice. * p <0.05, ** p <0.01, *** p <0.001.

    Article Snippet: BRCA cell lines HCC-1937, MCF-10A, MCF-7, BT474, SK-BR-3, MDA-MB-468, BT-549, and MDA-MB-231 were purchased from Procell Life Science & Technology Co., Ltd., Wuhan, China.

    Techniques: Over Expression, Expressing, Migration, Knockdown